Translational_Unit

Part:BBa_K1472612:Design

Designed by: Choi hiu Ying, LEE Cheuk Lam, Tse Yui Shing, Yeung Sin Yu   Group: iGEM14_CityU_HK   (2014-09-29)


Delta 9,12 and 15 Desaturase


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 2095
    Illegal suffix found in sequence at 3198
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2095
    Illegal SpeI site found at 3199
    Illegal PstI site found at 3213
    Illegal NotI site found at 2101
    Illegal NotI site found at 3206
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2095
    Illegal BglII site found at 1070
    Illegal BamHI site found at 465
    Illegal BamHI site found at 1430
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 2095
    Illegal suffix found in sequence at 3199
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 2095
    Illegal XbaI site found at 2110
    Illegal SpeI site found at 3199
    Illegal PstI site found at 3213
    Illegal AgeI site found at 289
    Illegal AgeI site found at 1251
    Illegal AgeI site found at 1515
    Illegal AgeI site found at 1653
    Illegal AgeI site found at 2508
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The desaturase genes in plasmids were introduced into E. coli for amplification. The final construct includes iGEM provided RBS (BBa_B0034).


Source

All the desaturase sequences are derived from the Synechocystis sp. but optimised for the expression in E. coli.

References